Abstract
Objective: Evaluate the sterility of silicone tubes after sterilization in autoclave of saturated vapor under pressure before and after intervention in the cleaning stage. Method: It is a quasi-experimental study performed in a College hospital, in the municipality of Goiania, Brazil. 120 segments were analyzed: 60 in the extremity (E) and 60 in the middle (M). Group (1): 30 tubes, before the cleaning stage and group (2): 30 tubes after this intervention. Samples were inoculated, peaked in nutrient agar and the colonies were isolated and identified. Results: in group (1), 23 (76,67%) were contaminated and in the group (2) eight (26,67%). There was a significant statistical difference between the groups (X2 = 25699, p = 0,0001) and not between the segments. Prevailing microorganisms were: Staphylococcus coagulase-negative. Discussion: the significant statistical difference between the groups related to microbial development, reveals interference in the cleaning stage of reprocessing. Conclusion: the cleaning stage is fundamental for the success of reprocessing
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